Genetics From Genes to Genomes Study Set 1

Biology

Quiz 9 :

Digital Analysis of Dna

Quiz 9 :

Digital Analysis of Dna

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How many clones would make up one genomic equivalent?
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C

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Which is a characteristic of a plasmid used as a cloning vector? (Select all that apply. )
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A, B, C

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In Sanger sequencing, what causes DNA synthesis to terminate at a specific base?
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D

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What is the purpose of ethidium bromide in DNA electrophoresis?
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In Sanger sequencing, the role of the DNA polymerase is to
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What is the minimum number of clones that should be in this mouse genomic library to have a 95% chance that it contains the entire genome?
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A mouse genomic library is constructed so that the number of clones equals one genomic equivalent.Is it likely that this library contains the entire mouse genome?
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You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace. img -What is the sequence of the template DNA used for this sequencing reaction?
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Which is an example of a recombinant DNA molecule?
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You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace. img -What is the sequence of the DNA synthesized in the sequencing reaction?
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You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace. img -Which is a step in hierarchical genome sequencing that is not found in shotgun genome sequencing?
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Why isn't bacterial DNA digested by the restriction enzymes bacteria produce?
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In gel electrophoresis, DNA fragments move at different rates because they have different
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Imagine that DNA is composed of equal proportions of six different types of nucleotide, instead of the normal four.The six nucleotides are A, C, G, T, X, and Y.In this new type of DNA molecule, A pairs with T, C pairs with G, and X pairs with Y.What would be the average size of the DNA fragments produced when this new DNA is cut by a restriction endonuclease with a four-base recognition sequence?
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What is the function of the ampr gene in a plasmid vector?
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The recognition site for a restriction enzyme is 5′ ACC^GGT 3′.The ^ symbol indicates the site of cleavage.After digestion of DNA with this restriction enzyme, how will the ends of the resulting DNA fragments look?
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The recognition site for a restriction enzyme is 5′ ACCGG^T 3′.The ^ symbol indicates the site of cleavage.After digestion of DNA with this restriction enzyme, how will the ends of the resulting DNA fragments look?
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Which vector would be best to use if you want to construct a genomic library with the largest fragments possible?
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What is one difference between using restriction endonucleases and mechanical shearing of DNA?
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What is the origin and physiological function of restriction endonucleases?
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