Genetics From Genes to Genomes Study Set 1
Quiz 17 :
Gene Regulation in Eukaryotes
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Q08 Q08 Q08
A putative regulatory sequence is fused to an enhancerless GFP gene in a reporter construct and that construct is then used to generate a transgenic organism.If the putative regulatory sequence contains an enhancer that binds with an eye-specific combination of activators, how will the transgenic organism look?
Q33 Q33 Q33
Gene B is usually expressed only in skin cells.To learn about the mechanism by which expression of the gene B is regulated, you make clones that contain a GFP reporter and various parts of the upstream and downstream intergenic regions of genomic DNA that normally surround gene B (black lines)as shown in the figure below.The resulting clones were introduced into frog skin cells growing in the lab and levels of GFP expression was monitored by measuring green fluorescence.The table at left shows relative GFP expression, with more +s equal to more expression.(one + = basal expression). -Interactions between an enhancer and a repressor initiates a series of events that result in a change in gene expression.In what order can the events occur? (Some steps may not be used.Put the remaining steps in chronological order from first to last. ) 1.Transcription of the gene of interest is reduced. 2.A corepressor is recruited. 3.Chromatin around the gene of interest expands. 4.Histone tails may be methylated or deacetylated. 5.Chromatin around the gene of interest becomes more compact. 6.Proteins attached to the promoter and enhancer associate with each other and DNA bends.
Q35 Q35 Q35
This pedigree shows the inheritance pattern of a disease caused by a paternally imprinted allele in a family.Genetic testing of individuals I-1 and II-5 indicates they are carriers of the disease allele.No one else in the family agreed to be tested.If first cousins III-4 and III-5 have a child together (IV-1)what is the chance the child will have the disease? (assume complete penetrance) Reference for pedigree: Modified from www.cell.com/trends/molecular-medicine/fulltext/S1471-4914(14)00138-5