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Genetics From Genes to Genomes Study Set 1

Biology

Quiz 18 :

Manipulating the Genomes of Eukaryotes

Quiz 18 :

Manipulating the Genomes of Eukaryotes

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If integration of DNA provided by pronuclear injection occurs after three cell divisions, the resulting mouse will
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B

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What is the purpose of the P element in the creation of transgenic Drosophila?
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A

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What is a possible disadvantage of using genetically modified crops?
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A

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The coding sequence of the green fluorescent protein gene (GFP)is placed under the control of an enhancer active in neurons.When this transgene is introduced into flies, GFP will be expressed in
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What is one problem associated with using retroviruses as human gene therapy vectors?
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Why are conditional knockout animals desirable?
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Knockin mice can be created using the Cre/loxP system.In this procedure, the loxP sites are used to
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Transgenic organisms are used to clarify which gene may be responsible for a mutant phenotype because
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Recombinant Ti plasmids allow for
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After exposure to Agrobacterium tumefaciens bacteria containing both a recombinant T-DNA vector and a helper plasmid, new plants can be grown from single cells in the presence of herbicide.Without the herbicide,
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To create transgenic mice, DNA is injected into
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CRISPR sequences occur naturally as
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How can transgenic organisms be used to identify transcriptional enhancers?
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Currently the two main vectors for delivery of therapeutic genes for human gene therapy are
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Transgenic animals made by pronuclear injection can be limited for studies of the links between genes and disease because
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A scientist wants to determine whether a mutant phenotype is due to the loss of gene C or gene D, which are both deleted in a mutant fly with abnormal eyes.Mutant flies with transgenic gene C have wild-type eyes, whereas mutant flies with transgenic gene D have abnormal eyes.What can be concluded from these results?
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Pharming refers to the use of transgenic animals and plants to produce protein drugs, such as
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A trait unique to embryonic stem cells that makes them useful for generating knockout animals is that they
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Although bacteria can be used to produce proteins for therapeutic uses, what can be a disadvantage to using a prokaryotic system?
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A researcher has a laboratory strain of flies that have loss-of-function mutations in both gene X and white.These flies are used as hosts for P element transformation: Embryos of the double mutant strain are injected with two plasmids, one containing a wild-type copy of the white gene (w+)and a wild-type copy of gene X, side-by-side within P element inverted repeats, and the other plasmid containing only the P element transposase gene.Transgenic offspring of the injected flies will
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