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Genetics From Genes to Genomes Study Set 1

Biology

Quiz 14 :

Bacterial Genetics

Quiz 14 :

Bacterial Genetics

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Horizontal gene transfer
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A

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What characteristics make bacteria like E.coli attractive as model organisms?
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B

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If an organism is isolated that has no nuclear membrane and the DNA is condensed into a body in the cytoplasm, this organism would most likely be classified as
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Some plasmids can replicate in several distinct species of bacteria.Frequently these plasmids have transposons carrying several different antibiotic resistance genes.In a hospital that has an outbreak of several species of bacteria each carrying resistance to three drugs (streptomycin, gentamicin, and penicillin), what is the best way to determine whether or not this resistance is due to a single shared plasmid with all three resistance genes?
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Metagenomics may be useful because
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Which gene would you predict would be most likely to be part of a core genome and not the pangenome?
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Viruses are isolated from wild-type E.coli cells that have been infected with wild-type bacteriophage λ.These viruses are used to infect a Gal strain of E.coli.A few bacterial colonies that can grow on galactose are obtained, while no bacteria that can grow on galactose are obtained from cells that were not infected.What has happened?
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In an Hfr strain, the F episome is integrated between genes A and B.When this Hfr mates with an F strain, gene B is always transferred first to the F cell.What is the last bacterial gene that could possibly (at least in theory)be transferred to the F from the host chromosome?
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The episome in an Hfr strain is inserted near the trp (tryptophan operon)locus.This Hfr strain is grown with an F- Trp- strain.From this mating, we isolate an F+ Trp+ strain that can readily impart the Trp+ phenotype to F- Trp- strains, but no other traits are ever transferred.(When the original Hfr strain is mated with F strains, traits other than Trp+ can be transferred, although at lower frequency than Trp+. )What most likely has happened?
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What would you anticipate would be true about the metabolism of a bacterial species isolated from water samples, one at the surface and another from a hot vent on the ocean floor?
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A bacterium is found that is resistant to the antibiotic gentamicin.The bacterium was isolated in a hospital where patients were routinely given gentamicin for a variety of infections.What was the pressure that selected for this resistant population?
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Which technique would most likely be used for mapping genes that are separated by 2 × 105 base pairs?
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Two F+ E.coli strains are co-cultured in the same flask.One is Strr Thr+ and the other is Genr Thr.The culture is then plated on minimal media that is supplemented with gentamycin only (not threonine)and a few colonies grow.What sort of genetic exchange is most likely occurring?
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What is a difference between a Tn and IS element?
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What is a typical characteristic of bacterial chromosomes?
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If the Tn10 transposon is used to mutagenize a bacterium for the purpose of creating a mutation in the his operon, what would be the most appropriate selection media?
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Penicillin stops bacterial cell wall formation by
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What is a property of a pathogenic bacterium?
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What would happen if a transposon were to integrate into the replication origin of the bacterial genome?
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A strain of E.coli is trp− his− lac−.Which medium would this bacterium grow on?
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