Quiz 13: Chromatin Structure and Its Effect on Transcription
Biology
Q 1Q 1
At least 20% of all amino acids found in histones are the basic amino acids
A)glycine or serine.
B)arginine or lysine.
C)tryptophane or tyrosine.
D)valine or praline.
E)cysteine or isoleucine.
Free
Multiple Choice
B
Q 2Q 2
Which of the following histone proteins is the most highly conserved from one organism to another?
A)H1
B)H2A
C)H2B
D)H3
E)H4
Free
Multiple Choice
D
Q 3Q 3
Which of the following is the most common type of histone modification?
A)lysine -amino methylation
B)serine o-phosphorylation
C)lysine n-phosphorylation
D)histidine n-phosphorylation
E)acetylation
Free
Multiple Choice
D
Q 4Q 4
One method used by researchers to demonstrate the existence of nucleosome free DNA regions is
A)RNA Polymerase Run-off experiments.
B)Nuclear Run on experiments.
C)Reporter gene experiments.
D)Anion Exchange chromatography.
E)DNase hypersensitivity experiments.
Free
Multiple Choice
Q 5Q 5
Histone acetylation can occur in the
A)nucleus.
B)cytoplasm.
C)ER lumen.
D)nucleus and cytoplasm.
E)nucleus,cytoplasm,and ER lumen.
Free
Multiple Choice
Q 6Q 6
Which of the following molecules can expedite RNA Pol II elongation through a nucleosome?
A)HAT
B)FACT
C)IFN-
D)ChIP
E)TRE
Free
Multiple Choice
Q 7Q 7
Which of the following is the correct order of chromatin folding?
A)nucleosome formation,30 nM fiber formation,radial loop structure
B)radial loop structure,30 nM fiber formation,nucleosome formation
C)30 nM fiber formation,nucleosome formation,radial loop formation
D)30 nM fiber formation,radial loop formation,nucleosome formation
E)nucleosome formation,radial loop formation,nucleosome formation
Free
Multiple Choice
Q 8Q 8
The Histone Code states that the
A)primary sequence of the histone proteins never changes over time.
B)combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of that gene.
C)combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of all the nearby genes.
D)lysines are the only amino acids found in histones that can be acetylated and deacetylated.
E)histones are found in all living cells.
Free
Multiple Choice
Q 9Q 9
Some transcription factors,such as ________ can act as both antirepressors and transcription activators.
A)GAL4
B)Rad6
C)SIR2
D)RAP1
E)HP1
Free
Multiple Choice
Free
Short Answer
Q 11Q 11
The histone protein _______ is more readily removed from chromatin than the other histone proteins and therefore is not considered part of the core nucleosome.
Free
Short Answer
Free
Short Answer
Q 13Q 13
Acetylation of histones occurs on the amino groups of the side chains of the amino acid __________.
Free
Short Answer
Q 14Q 14
Deacetylation of core histones allows for stronger binding of histones to DNA,which then inhibits ___________________.
Free
Short Answer
Free
Short Answer
Q 16Q 16
Antirepressors such as GAGA factor are thought to compete with ________ for binding sites on the DNA template.
Free
Short Answer
Free
Short Answer
Free
True False
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True False
Q 20Q 20
Chromatin remodeling usually occurs on euchromatin,while heterochromatin is very condensed and genetically inactive.
Free
True False
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True False
Q 22Q 22
If histone acetylation occurs in the cytoplasm,then histone deacetylation will also occur in the cytoplasm.
Free
True False
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True False
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True False
Q 25Q 25
When added to DNA in vitro,unacetylated histones tend to increase transcription from the 5S rRNA promoter.
Free
True False
Q 26Q 26
In nucleosome positioning,activators force the nucleosome to take up positions around the promoter so RNA polymerase can bind.
Free
True False
Q 27Q 27
Chromatin immunoprecipitation (ChIP)can be used to study chromatin methylation in yeast cells.
Free
True False
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True False
Q 29Q 29
Layborne and Kadonage demonstrated in vitro that by adding increasing amounts of core histones and H1 they could repress transcription from the Drosophila Krüppel gene.
Free
True False