Quiz 22: Homologous Recombination
Biology
Q 1Q 1
Which of the following statements is not true concerning homologous recombination?
A)It can be intramolecular.
B)It can be bimolecular.
C)Only one cross-over event can occur.
D)It results in genetic variation.
E)It is a reciprocal event.
Free
Multiple Choice
C
Q 2Q 2
Which of the following techniques was key in demonstrating the presynapsis and synapsis steps of homologous recombination?
A)S1 nuclease mapping
B)exonuclease digestion
C)DNA footprinting
D)Southern Blot
E)electron microscopy
Free
Multiple Choice
E
Q 3Q 3
Molecular modeling of RuvA and a Holliday junction showed that they form a _________ that favors rapid branch migration.
A)square planar conformation
B)L-shape conformation
C)clover leaf conformation
D)D loop conformation
E)Y-shape conformation
Free
Multiple Choice
A
Q 4Q 4
West and colleagues used gel mobility shift assays to demonstrate that __________ could bind to and resolve Holliday junctions.
A)RecA
B)RuvA
C)RuvB
D)RuvC
E)RecB
Free
Multiple Choice
Q 5Q 5
One mechanism by which gene conversion can occur in N.crassa during meiosis is _______.
A)RecBCD
B)mismatch repair
C)double stranded break synthesis
D)SOS repair
E)excision repair
Free
Multiple Choice
Q 6Q 6
________________ recombination is a cross-over event that joins previously separated DNA segments.
Free
Short Answer
Q 7Q 7
The E.coli genome,on average,contains approximately __________chi sites that can be involved in homologous recombination.
Free
Short Answer
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Short Answer
Q 9Q 9
During postsynapsis strand exchange in homologous recombination of E.coli,ATP is necessary for _______ clearance from the synapsing DNA.
Free
Short Answer
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Short Answer
Q 11Q 11
A ________ double helix is different than a Watson-Crick double helix in that the two strands are aligned side by side instead of intertwined.
Free
Short Answer
Q 12Q 12
RuvA and RuvB are involved in ________ of a Holliday junction during homologous recombination.
Free
Short Answer
Q 13Q 13
________resolvase clips DNA at the consensus sequence,5-(A/T)TT(G/C)-3 during RecBCD recombination repair.
Free
Short Answer
Q 14Q 14
In eukaryotes,_______ often includes homologous recombination as a way to increase genetic variation.
Free
Short Answer
Q 15Q 15
During mating between two strains of Neurospora crassa containing different alleles of the same gene one would expect a 1:1 genotypic ratio in progeny spores,however,approximately 0.1% of the time this ratio strays from the predicted.This phenomenon is called_____________ ___________________.
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Short Answer
Q 16Q 16
Spo11 creates __________ in DNA during homologous recombination in Saccharomyces cerevisiae.
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Short Answer
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True False
Q 18Q 18
Branch migration is a spontaneous process that takes place after a Holliday junction is formed.
Free
True False
Q 19Q 19
The role of SSB in RecBCD homologous recombination appears to be to unwind the secondary structures found in dsDNA.
Free
True False
Q 20Q 20
David Gonda and Charles Radding have provided experimental evidence that the minimum degree of homology necessary for efficient synapsis to occur during homologous recombination is between 30 and 151 bp.
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True False
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True False
Q 22Q 22
Since RecA is such a versatile protein,RecBCD is not really necessary for homologous recombination to occur.
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True False
Q 23Q 23
ATPS is an unhydrolyzable ATP analog that was used along with glutaraldehyde by Parsons and West to trap Holliday junctions for gel mobility shift assays.
Free
True False
Q 24Q 24
Both RuvA and RuvB have ATPase activity that drives the unwinding and rewinding of DNA during branch migration.
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True False
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True False
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True False
Q 27Q 27
A conserved tyrosine residue is likely to be the catalytic amino acid in Spo11 and homologous proteins.
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True False
Q 28Q 28
Double stranded break formation in mitotic recombination is followed by 35 exonuclease digestion of 3 ends at the break to yield 5 overhangs.
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True False
Q 29Q 29
All organisms undergo homologous recombination as a way to increase genetic variation and help cope with DNA damage.
Free
True False
Q 30Q 30
Gene conversion usually only occurs between nonallelic genes such as the MAT genes used to determine mating type in yeasts.
Free
True False