Quiz 14: Rna Processing I: Splicing
Biology
Q 1Q 1
Which of the following is not true concerning eukaryotic mRNA processing?
A)addition of a 3 poly-A tail
B)addition of a 5 cap
C)intron splicing
D)occurs in the nucleus
E)occurs in the cytoplasm
Free
Multiple Choice
E
Q 2Q 2
Which of the following experiments was useful in determining the existence of introns in the adenovirus genome?
A)DNA/DNA hybridization
B)RNA/RNA hybridization
C)R-looping experiments
D)RT-PCR
E)cDNA cloning experiments
Free
Multiple Choice
C
Q 3Q 3
You have isolated a new species of mRNA using an oligo-dT column.You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns.Below is a representation of the electron micrograph results.Which of the following can you conclude from your experiment?
A)The gene contains no introns.
B)The gene contains one intron.
C)The gene contains two introns.
D)The gene contains three introns.
E)The results are inconclusive.
Free
Multiple Choice
C
Q 4Q 4
The first two bases and the last two bases in the splicing signal consensus sequence are
A)GT-AG.
B)GU-AG.
C)CU-AG.
D)GU-AC.
E)GT-TG.
Free
Multiple Choice
Q 5Q 5
Which of the following is the name for the Yeast 40S particle where mRNA splicing takes place?
A)ribosome
B)nucleolus
C)spliceosome
D)hnRNA
E)R-loop
Free
Multiple Choice
Free
Multiple Choice
Q 7Q 7
Which of the following snRNP is mismatched with its function?
A)U1: base pairs with 5 splice site of mRNA.
B)U2: base pairs with the conserved sequence at splicing branch point.
C)U4: base pairs with 3 splice site of mRNA.
D)U5: associates with last nucleotide in one exon and the first nucleotide in the next exon.
E)U6: base pairs with 5 end of the intron.
Free
Multiple Choice
Q 8Q 8
Which of the following complexes found in the spliceosome cycle is mismatched with its snRNP(s)?
A)commitment complex: U6/U4
B)A complex: U1 and U2
C)B1 complex: all snRNPs
D)B2 complex: U2,U5 and U6
E)I complex: U2,U5 and U6
Free
Multiple Choice
Q 9Q 9
Which of the following splicing factors is required for the correct 3 splice site selection?
A)SC35
B)Slu7
C)U11
D)SF2
E)SRp55
Free
Multiple Choice
Q 10Q 10
The catalytic center of the spliceosome appears to include
A)Mg2+.
B)U2 and U6 snRNP.
C)the branch point region of the intron.
D)Mg2+ and the branch point region of the intron.
E)Mg2+,U2,and U6 snRNP,and the branch point region of the intron.
Free
Multiple Choice
Q 11Q 11
Which of the following is the first snRNP to bind during the assembly stage of the spliceosome cycle?
A)U1
B)U2
C)U4
D)U5
E)U6
Free
Multiple Choice
Q 12Q 12
The binding of which of the following snRNPs to spliceosome requires ATP?
A)U1
B)U2
C)U4
D)U5
E)U6
Free
Multiple Choice
Q 13Q 13
The expressed regions of genes are referred to as ________ while the noncoding regions are called_______.
Free
Short Answer
Free
Short Answer
Q 15Q 15
_______________ disrupting the normal splicing consensus sequence are one way to obtain abnormal splicing patterns.
Free
Short Answer
Q 16Q 16
Joan Steitz was one of the researchers who used __________ studies to demonstrate that U6 snRNP binds to the 5 end of the intron.
Free
Short Answer
Q 17Q 17
Some introns,such as Group II introns,are self splicing.These types of introns contain catalytic RNA's,also referred to as __________.
Free
Short Answer
Free
Short Answer
Free
Short Answer
Free
Short Answer
Free
True False
Free
True False
Free
True False
Free
True False
Q 25Q 25
Experimental evidence demonstrates that mRNA precursors are spliced via a lariat-shaped intermediate.
Free
True False
Q 26Q 26
Base pairing between the 5 splice site and U1 snRNA is the only interaction essential for splicing.
Free
True False
Free
True False
Free
True False
Q 29Q 29
Alternative splicing patterns can lead to the production of different protein products from the same gene.
Free
True False
Q 30Q 30
SC35 is an RNA binding protein that is responsible for the commitment to splice the -globin pre-mRNA.
Free
True False
Free
True False