Quiz 16: Rna Processing: Other Post-Transcriptional Events

Which of the following enzymes removes extra nucleotides from the 5 ends of pre-tRNA? A)RNase I B)RNase II C)RNase III D)RNase E E)RNase P

Most cellular RNase P enzymes have 2 subunits,one protein subunit and I RNA subunit.RNase P from which of the following has no known RNA subunit? A)human nuclei B)monkey mitochondria C)spinach chloroplast D)yeast nuclei E)Escherichia coli

In which of the following organisms is trans-splicing of mRNA known to occur? A)trypanosomes B)yeast C)bacteria D)mammals E)plants

Trypanosome mitochondria contain two types of circular DNA linked together into large networks.These mitochondria are called A)maxicircles B)kinetoplasts C)leucoplasts D)plastids E)maxi-mitochondria

Which of the following RNAs is involved in mRNA editing? A)rRNA B)tRNA C)gRNA D)snRNA E)iRNA

Which of the following techniques was used by Stuart and colleagues to demonstrate mRNA editing? A)RT-PCR B)cDNA cloning C)Northern Blot D)RNase footprinting E)mutation analysis

RNA editing by nucleotide deamination can occur in which of the following cell types? A)human brain B)HIV infected human cells C)red blood cells D)Drosophila E)All of the choices have RNA editing.

The function of normally occurring mRNA deamination appears to be the proper A)cleanup of the mRNA. B)mRNA folding. C)mRNA splicing. D)mRNA modification. E)protein synthesis.

Which of the following processes occurs posttranscriptionally? A)Cap addition B)adenosine deamination C)Poly(A)addition D)promoter clearance E)unwinding

Under which of the following conditions does TfR mRNA degradation take place? A)conversion of citrate to isocitrate B)IRE hairpin loop formation C)aconitase apoprotein binding to IRES D)low iron concentrations E)high iron concentrations

RNA interference occurs naturally in human cells during A)RNA viral replication. B)DNA viral replication. C)RNA viral transcription. D)DNA viral transcription. E)mRNA translation.

Which of the following enzymes is not involved in RNA interference? A)Dicer B)Slicer C)Argonaute D)Argonaute2 E)RNase P

Induces degradation of the target mRNA B

Usually formed from foreign RNAs B

Usually starts as part of the stem of a naturally occurring cellular RNA stem loop A

Usually base pair imperfectly with their target mRNA A

rRNA gene transcription occurs in the ______________.

________-splicing is the process by which exons from different genes are spliced together to form the mature mRNA.

Trypanosome mRNA editing occurs in the ______________.

If you were to treat mammary gland tissue with prolactin you would expect to see an increase in casein mRNA __________________.

MicroRNAs function to silence gene expression primarily by blocking ____________ of targeted mRNAs.

miRNA synthesis involves the cleavage of the double stranded stem portion of a precursor RNA by the enzyme ____________.

_________ RNA is responsible for RNA interference.

RNA editing in mammals involves deamination of adenosine to form __________.

Processing a pri-miRNA to a mature miRNA is a two-step process.

Transposition of transposons is blocked in animal germ cells by a ping-pong and mRNA destruction mechanism involving piRNAs.

E.coli rRNA is transcribed and processed in the cytoplasm.

Inactivation of RNase genes involved in tRNA 3 end maturation is lethal to bacterial cells.

mRNA editing has been shown to occur in the 5 to 3 direction.

Flowering plants have two nuclear RNA RNA polymerases,Iv and V,that are not found in animals and fungi.

Cellular mRNA levels often correlate more closely with transcription rate than with transcript stability.

Dicer is an RNase III-like enzyme that cleaves dsRNA into 50-55 nt fragments.

Gene silencing by RNAi can target the control region of a gene by being involved in DNA methylation.

Post-transcriptional processing of RNA is not important for gene expression.

Post-transcriptional processing of RNA occurs only in eukaryotic cells.