Question 1

The best tool available for the separation of many proteins at once is called ________.

Accepted Answer

2-D gel electrophoresis

Question 2

High density whole chromosome transcriptional mapping studies have shown that the majority of sequences in cytoplasmic polyadenylated RNAs derive from non-____________ regions of ten human chromosomes.

Accepted Answer

exon

Question 3

DNA microarrays can be used to study

Accepted Answer

DNA microarrays allow researchers to measure the expression of many different genes at once, providing information on the clustering of gene expression in time and space. They do not directly provide information on DNA sequence or chromosomal rearrangements or abnormalities.

Question 4

Which of the following is not a finding from analysis of the complete DNA sequence of human chromosome 22?

Accepted Answer

The finding that human chromosome 22 contains very little noncoding DNA is actually a result from analysis of the complete DNA sequence. Therefore, it is not the answer to this question. The other options are all findings from the analysis of the complete DNA sequence of human chromosome 22.

Question 5

A _____________ is the sum total of all the RNA transcripts in an organism at one time.

Accepted Answer

The answer of A _____________ is the sum total of...

Question 6

The NCBI website contains a vast store of biological information.If you have a sequence that you would like to compare to other known sequences,what is the name of the NCBI database that would allow such a search? ________.

Accepted Answer

The answer of The NCBI website contains a vast store...

Question 7

One place to find useful databases for bioinformatics is the _________ website.

Accepted Answer

The answer of One place to find useful databases for...

Question 8

Which of the following processes allows scientists to determine which genes are expressed in a given tissue and the extent of that expression?

Accepted Answer

SAGE (Serial Analysis of Gene Expression) is a process that can be used to determine which genes are expressed in a given tissue and to what extent. It involves the production of short sequence tags from expressed genes which are then sequenced and compared to a reference database to determine the level of expression. This technique is particularly useful for identifying genes that are differentially expressed between samples and for studying complex biological processes such as development and disease.

Question 9

Computational biology techniques allowed scientists to discover highly conserved sequence motifs in the promoter regions and ___________ of four mammalian species.

Accepted Answer

The answer of Computational biology techniques allowed scientists to discover...

Question 10

Which of the following processes allows scientists to identify the 5'-ends of transcripts?

Accepted Answer

CAGE (Cap Analysis of Gene Expression) is a method used to identify the 5’-end caps of transcripts, allowing for accurate mapping of transcription start sites. SAGE (Serial Analysis of Gene Expression) is a method used to quantify gene expression, but it does not specifically identify the 5’-ends of transcripts. RNAi analysis and oligonucleotide arrays are methods used to study gene function and expression, but do not specifically identify transcription start sites. PCR (Polymerase Chain Reaction) can amplify specific DNA sequences, but it does not specifically identify the 5’-ends of RNA transcripts.

Question 11

"Knocking out" genes is laborious.A simpler alternative to 'knock down' gene expression is called _____ ____________.

Accepted Answer

The answer of "Knocking out" genes is laborious.A simpler alternative...

Question 12

The sum total of all proteins produced by an organism is called its____________.

Accepted Answer

The answer of The sum total of all proteins produced...

Question 13

In one kind of mutation analysis called,__________ __________,mutants are created by replacing genes one at a time with antibiotic resistance genes flanked by oligomers that serve as a barcode to identify each mutant.

Accepted Answer

The answer of In one kind of mutation analysis called,__________...

Question 14

Genomic sequence information can be applied to study which of the following?

Accepted Answer

Genomic sequence information can be applied to study the evolution, function, and structure of genomes as well as for studying proteomics, therefore all of the choices are correct.

Question 15

The underlying strategy of the technique _________ is to synthesize short cDNAs,or tags,from all the mRNAs in a cell,and then to link these tags together in clones that can be sequences to learn the nature of the tags,and therefore the nature of the genes expressed in the cell.

Accepted Answer

The answer of The underlying strategy of the technique _________...

Question 16

Which of the following is not a technique that can be used to study protein-protein interactions?

Accepted Answer

2-D gel electrophoresis is a technique used to separate and analyze proteins based on their size and charge. However, it is not specifically designed to study protein-protein interactions. The other choices, such as protein microarrays, immunoaffinity chromatography, phage display, and yeast two-hybrid analysis, are methods specifically used to investigate protein-protein interactions.

Question 17

Which of the following prcoesses allows scientists to determine the expression patterns (temporal and spatial)of many genes at once?

Accepted Answer

DNA microarray technology allows scientists to simultaneously measure the expression levels of thousands of genes in a single experiment. This provides insight into the temporal and spatial patterns of gene expression. SAGE and CAGE are also gene expression profiling techniques, but they do not allow for the simultaneous measurement of multiple genes like microarrays do. Genome sequencing and RNAi analysis do not specifically measure gene expression patterns.

Question 18

Which of the following techniques was developed by Velculescu and colleagues to determine which genes are expressed in a given tissue?

Accepted Answer

SAGE (Serial Analysis of Gene Expression) was developed by Velculescu and colleagues to determine which genes are expressed in a given tissue. It involves sequencing short tags of mRNA to identify which genes are being actively transcribed. This technique allows for more accurate and quantitative measurements of gene expression compared to previous methods such as Northern blotting or RT-PCR.

Question 19

Tissue-specific expression profiling can be done by examining the spectrum of mRNAs whose levels are decreased by an exogenous _________ and comparing that to the spectrum of expression of genes at the mRNA level in various tissues.

Accepted Answer

The answer of Tissue-specific expression profiling can be done by...

Question 20

Which of the following processes allows scientists to 'knock-down' genes?

Accepted Answer

RNAi analysis (RNA interference) allows scientists to 'knock-down' genes by introducing small interfering RNAs (siRNAs) that bind to and degrade the mRNA produced by the target gene, thus reducing its expression. SAGE (Serial Analysis of Gene Expression) and CAGE (Cap Analysis of Gene Expression) are methods for quantifying gene expression levels, while CHIP-chip and DNA microchip are techniques for analyzing protein-DNA interactions.

Quiz 25: Genomics II: Functional Genomics,proteomics and Bioinformatics