Quiz 24: Introduction to Genomics: Dna Sequencing on a Genomic Scale
Biology
Q 1Q 1
Which of the following enzymes is used to search for CpG islands?
A)EcoRI
B)HindIII
C)HpaII
D)PvuI
E)BamHI
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Multiple Choice
C
Q 2Q 2
Which of the following processes was used to locate the gene responsible for Huntington disease?
A)shot-gun cloning
B)positional cloning
C)genome sequencing
D)SAGE
E)proteomics
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Multiple Choice
B
Q 3Q 3
Which of the following free living organisms has the smallest genome,containing about only 470 genes?
A)Haemophilus influenzae Rd
B)Mycoplasma genitalium
C)Methanococcus jannaschii
D)Saccharomyces cerevisiae
E)Caenorhabditis elegans
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Multiple Choice
B
Q 4Q 4
One of the first steps in the Clone-by-Clone strategy is to map the genome using chromosomal markers.Which of the following is not a marker used to map a genome?
A)YACs
B)RFLPs
C)VNTRs
D)STSs
E)ESTs
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Multiple Choice
Q 5Q 5
Which of the following is not a finding from analysis of the complete DNA sequence of human chromosome 22?
A)It contains 679 annotated genes.
B)It contains local and long range duplications.
C)It contains orthologs to the mouse chromosome.
D)It contains very little noncoding DNA.
E)It contains "poison regions" or gaps that may never get sequenced.
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Multiple Choice
Q 6Q 6
The estimated total number of genes in the human genome is
A)10,000-15,000.
B)20,000-25,000.
C)40,000-50,000.
D)50,000-75,000.
E)100,000-130,000.
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Multiple Choice
Q 7Q 7
Which of the following types of genetic markers tend to bunch together at the ends of chromosomes?
A)VNTRs
B)a class of STSs called microsatellites
C)STSs
D)RFLPs
E)none of the above
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Multiple Choice
Q 8Q 8
The working draft of the human genome reported that the genome contains approximately how many genes?
A)less than 1,000
B)2,000
C)5,000
D)10,000
E)20,000
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Multiple Choice
Q 9Q 9
Comparison of the genomes of humans and our closest living relative,the chimpanzee,has identified a few DNA regions that have changed rapidly since the two species diverged.Which statement below accurately depicts the thing that sets humans apart from chimpanzees.
A)the few regions that have rapidly changed are all protein-encoding genes
B)humans have been found to have many more genes than chimpanzees
C)chimpanzees have been found to have many more non-coding regions in their genome
D)the control of the genes,rather than the genes themselves are what sets humans apart from chimpanzees
E)all of the above
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Multiple Choice
Q 10Q 10
The fact that the genomes of two different individuals will produce fragments of different lengths when cut with restriction enzymes is called____________.
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Essay
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Short Answer
Q 12Q 12
Using RFLP geneticists were able to map the gene for Huntington disease to a region near the end of human chromosome ________.
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Short Answer
Q 13Q 13
The process by which a library of clones with different size inserts is first DNA sequenced and then a computer is used to piece the DNA sequences together is called________.
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Short Answer
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Short Answer
Q 15Q 15
In 2010,Venter and colleagues introduced an entirely synthetic M.mycoides genome into M.capricolum cells.The success of this experiment ushered in a new era of ?
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Short Answer
Q 16Q 16
The number of genes in the human genome appears to be a lot less than expected.One way for a cell to express multiple proteins from one gene is by_________________.
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Short Answer
Q 17Q 17
An _____________ is a sequence of bases that,if translated in one reading frame,contains no stop codons for a relatively long distance.
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Short Answer
Q 18Q 18
Geneticists have discovered a class of STSs called ____________ that are highly polymorphic and useful as genetic markers in genetic mapping.
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Short Answer
Q 19Q 19
BAC vectors are based on the E.coli F plasmid,which is normally used by the bacteria for ________________.
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Short Answer
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True False
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True False
Q 22Q 22
BAC cloning vectors are much more useful than the pUC cloning vectors for genome sequencing because they can accept larger DNA fragments.
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True False
Q 23Q 23
Positional cloning begins with mapping studies to pin down the location of the gene of interest to a reasonable small region of DNA.
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True False
Q 24Q 24
In the context of genetic mapping,mapping depends on a set of landmarks to which the position of a gene can be related.Sometimes the landmarks are genes but more often they are RFLPs.
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True False
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True False
Q 26Q 26
Genome size is a good indication of the number of genes it contains,the larger the genome the greater the number of genes.
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True False
Q 27Q 27
Using M.genitalium,Ventor and colleagues have shown that the essential gene set for life is the same size as the minimal genome to sustain a cell.
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True False
Q 28Q 28
A gene-finding technique exists that takes advantage of the fact that control regions of active human genes tend to be associated with unmethylated CpG sequences,compared to the CpGs in inactive regions that are almost always methylated.
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True False
Q 29Q 29
Radiation hybrid mapping involves irradiation of human cells to break the chromosomes,then fusion of the dead cells with hamster cells to form hybrid cells.The further apart two markers are on a chromosome the more likely they will be found in two different hybrids.
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True False