Quiz 15: Rna Processing II: Capping and Polyadenylation
Biology
Q 1Q 1
Please put the following steps of Cap synthesis in the correct order.(1)N7 of the capping guanine is methylated.(2)The terminal phosphate is removed from the pre-mRNA.(3)A capping GMP is added to the pre-mRNA.(4)The 2-O-methyl group of the penultimate nucleotide is methylated.
A)1,2,3,4
B)1,4,3,2
C)2,4,1,3
D)2,3,1,4
E)4,3,2,1
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Multiple Choice
D
Q 2Q 2
When does capping of the pre-mRNA occur?
A)It is the first nucleotide added by RNA polymerase.
B)It occurs before the mRNA reaches a chain length of 30 nt.
C)It occurs after the poly(A)tail has been added.
D)It occurs after splicing has occurred.
E)It occurs after export of the mRNA into the cytoplasm.
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Multiple Choice
B
Q 3Q 3
Which of the following is NOT a function of the mRNA cap?
A)protects the mRNA from degradation
B)enhances translatability of the mRNA
C)enhances transport of the mRNA to the cytoplasm
D)enhances splicing of the mRNA
E)helps regulate expression of the mRNA
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Multiple Choice
E
Q 4Q 4
Although snRNA U1 functions in the nucleus,it receives a 5 cap to enhance its
A)binding to snRNPs.
B)export to the cytoplasm.
C)export to the ER lumen.
D)export out of the cell.
E)binding to the mature mRNA.
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Multiple Choice
Q 5Q 5
The fact that actinomycin D does not inhibit polyadenylation supports the fact that
A)polyadenylation occurs pre-transcription of mRNA.
B)polyadenylation occurs during transcription.
C)polyadenylation occurs post-transcription.
D)it happens in the nucleus.
E)it happens in the cytoplasm.
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Multiple Choice
Q 6Q 6
Which of the following is the mRNA polyadenylation signal motif found in mammalian cells?
A)AAGAAA
B)AAUAAA
C)AAUGGG
D)UUAUUU
E)GGAUUU
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Multiple Choice
Q 7Q 7
Which of the following is not required for mammalian cell polyadenylation of pre-mRNA?
A)PAP
B)CPSF
C)PABII
D)RNA polymerase I
E)RNA polymerase II
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Multiple Choice
Q 8Q 8
Which of the following regions on calf-thymus poly(A)polymerase has been shown experimentally not to be necessary for its activity in vitro?
A)RNA binding domain
B)polymerase module
C)nuclear localization signals
D)serine/threonine rich regions
E)All domains are necessary.
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Multiple Choice
Q 9Q 9
The CTD domain of which subunit of RNA Polymerase II has been shown to be involved in pre-mRNA processing?
A)Rpb1
B)Rpb2
C)Rpb4
D)Rpb6
E)Rpb12
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Multiple Choice
Q 10Q 10
The poly(A)tail of eukaryotic mRNA is approximately ___________ nucleotides in length.
A)5
B)25
C)100
D)250
E)1000
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Multiple Choice
Q 11Q 11
Poly(A)polymerase can be found in the _____________ of a cell.
A)nucleus
B)cytoplasm
C)lysosomes
D)nucleus and cytoplasm
E)nucleus and lysosomes
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Multiple Choice
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Short Answer
Q 13Q 13
The function of a nucleotide phosphohydrolase is to ______________ phosphates from its substrate.
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Short Answer
Q 14Q 14
The human vaccinia virus replicates in the _________ of cells and therefore must carry with it its own capping enzymes.
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Short Answer
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Q 17Q 17
At least 5 different cDNAs have been isolated for the enzyme poly(A)polymerase.These cDNAs are a result of alternative ___________ and ______________ of the poly(A)polymerase gene.
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Short Answer
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Short Answer
Q 19Q 19
The cap plays a role in the removal of the ___________ intron while poly(A)tail plays a role in the removal of the ____________ intron.
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Short Answer
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True False
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True False
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True False
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True False
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True False
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True False
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True False
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True False
Q 31Q 31
Experiments using yeast as a model have shown that cleavage of the poly(A)tail is necessary for transcription termination.
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True False