In the Southern blot technique,which of the following is NOT required?
A)transfer of DNA to nitrocellulose
B)addition of a labeled probe to identify the gene of interest
C)restriction enzyme digestion of DNA
D)electrophoresis to separate fragments
E)addition of heat-stable DNA polymerase to amplify DNA

Question

Quizplus · Accepted Answer

The addition of heat-stable DNA polymerase to amplify DNA is not required in the Southern blot technique. This technique involves transfer of DNA fragments from an electrophoresis gel to a solid support like nitrocellulose, followed by probe hybridization to identify the gene of interest. Restriction enzyme digestion of DNA is required to generate specific fragments which can be separated by electrophoresis.

In the Southern Blot Technique,which of the Following Is NOT