Figure 9.5

-In Figure 9.5,the marker genes used for selecting recombinant DNA are
A)HindIII,BamHI,and EcoRI.
B)ampᴿ and lacZ.
C)ori.
D)ampᴿ and ori.
E)lacZ and ori.

Question

Figure 9.5
 
-In Figure 9.5,the marker genes used for selecting recombinant DNA are
A)HindIII,BamHI,and EcoRI. 
B)ampᴿ and lacZ. 
C)ori. 
D)ampᴿ and ori. 
E)lacZ and ori.

Quizplus · Accepted Answer

The marker genes used for selecting recombinant DNA in Figure 9.5 are ampᴿ and lacZ. These genes are commonly used in plasmid vectors to allow for selection and screening of transformants. Ampᴿ (ampicillin resistance) provides resistance to ampicillin, allowing for selection of cells that have taken up the plasmid, while lacZ (β-galactosidase) allows for screening of cells that have successfully incorporated the DNA of interest by assessing their ability to metabolize certain substrates.

Figure 9.5 -In Figure 9.5,the Marker Genes Used for Selecting Recombinant DNA