Which of the following places the steps in the PCR procedure in the correct order?
1)Incubate at 94°C to denature DNA strands;
2)Incubate at 72°C for DNA synthesis;
3)Incubate at 60°C for primer hybridization.
A)1,2,3
B)3,2,1
C)1,3,2
D)2;1;3
E)3;1;2

Question

Quizplus · Accepted Answer

The PCR procedure starts with denaturing the DNA strands by incubating at 94°C (step 1), followed by annealing the primers to the target DNA sequence by incubating at 60°C (step 3). Finally, DNA synthesis takes place by the DNA polymerase enzyme at 72°C (step 2). Therefore, the correct order of the steps in the PCR procedure is 1, 3, and 2, which is given by option C.

Which of the Following Places the Steps in the PCR