The following steps must be performed to make a bacterium produce human protein X.
1-Translation
2-Restriction enzyme
3-Prokaryotic transcription
4-DNA ligase
5-Transformation
6-Eukaryotic transcription
7-Reverse transcription
Which of the following places the steps in the correct order?
A)5,2,3,4,7,6,1
B)1,2,3,5,4,7,6
C)6,7,2,3,4,5,1
D)6,7,2,4,5,3,1
E)6,2,1,3,4,5,7

Question

Quizplus · Accepted Answer

To produce a human protein in a bacterium, the process starts with eukaryotic transcription (6) of the human gene into mRNA, followed by reverse transcription (7) to create cDNA from the mRNA. This cDNA is then cut with restriction enzymes (2) and joined into a plasmid using DNA ligase (4). The plasmid is introduced into the bacterium through transformation (5), and the bacterium uses its machinery for prokaryotic transcription (3) and translation (1) to produce the human protein.

The Following Steps Must Be Performed to Make a Bacterium