Figure 9.5

-Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E.coli?
A)microinjection
B)transformation
C)gene guns
D)Ti plasmids and Agrobacterium

Question

Figure 9.5
 
-Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E.coli?
A)microinjection 
B)transformation 
C)gene guns 
D)Ti plasmids and Agrobacterium

Quizplus · Accepted Answer

The most common method used to introduce plasmids into E.coli is transformation. In transformation, the plasmid DNA is mixed with competent E.coli cells, and heat shock is used to facilitate the entry of plasmid DNA across the cell membrane. Microinjection is a method used for introducing DNA into individual cells, such as embryonic cells, but not for introducing DNA into bacteria. Gene guns and Ti plasmids with Agrobacterium are methods used for introducing DNA into plant cells, not bacteria.

Figure 9.5 -Which of the Following Methods Would Be Used to Introduce