Vectors for expressing a eukaryotic protein in a bacterium must contain
A) a eukaryotic promoter.
B) antibody recognition sequences.
C) an origin of replication.
D) EcoRI restriction enzyme recognition sites.

Question

Quizplus · Accepted Answer

Eukaryotic proteins cannot be expressed in bacteria without an origin of replication, which enables the vector to replicate in bacterial cells. The presence of a eukaryotic promoter would allow for transcription of the eukaryotic gene in bacterial cells, but this alone would not ensure expression without the necessary bacterial transcription factors and ribosomal machinery. Antibody recognition sequences would not be necessary unless the protein was being used for purification or detection purposes. EcoRI restriction enzyme recognition sites are also not necessary for expression of the protein.

Vectors for Expressing a Eukaryotic Protein in a Bacterium Must