Which vector would be used to express a human protein in E. coli?
A) An expression plasmid containing cDNA made from mRNA for the human protein
B) An expression plasmid containing an EcoRI-cut piece of the gene for the human protein
C) The human gene for the protein inserted into E. coli by a bacteriophage $\lambda$expression vector
D) A yeast artificial chromosome containing the human gene for the protein

Question

Quizplus · Accepted Answer

An expression plasmid containing cDNA made from mRNA for the human protein would be the best choice. cDNA is complementary DNA synthesized from an mRNA template, which lacks the introns present in genomic DNA, making it easier to express in a prokaryotic system like E. coli. The expression plasmid would contain regulatory elements like promoters and terminators to drive gene expression.

Which Vector Would Be Used to Express a Human Protein λ\lambdaλ

Which Vector Would Be Used to Express a Human Protein $\lambda$