The most common method of DNA sequencing is based on premature termination of DNA synthesis by inclusion of
A) dideoxynucleotides.
B) endonuclease.
C) ampicillin.
D) puromycin.

Question

Quizplus · Accepted Answer

The most common method of DNA sequencing is based on the Sanger method, which utilizes chain-terminating dideoxynucleotides (ddNTPs) to prematurely terminate DNA synthesis. These ddNTPs lack the 3'-OH group, which is necessary for further DNA strand elongation. As a result, incorporation of a ddNTP during DNA synthesis will halt the extension of the DNA strand, resulting in fragments of different lengths that can be separated by gel electrophoresis and sequenced. Endonucleases, ampicillin, and puromycin are not commonly used in DNA sequencing.

The Most Common Method of DNA Sequencing Is Based on Premature