Which pair of primers will amplify the template DNA sequence shown below in a PCR?
5ʹ-AGGCTGCGAT...//...CCGTTAGATT-3ʹ
3ʹ-TCCGACGCTA...//...GGCAATCTAA-5ʹ
A) 5ʹ-AGGCTGCGAT-3ʹ and 5ʹ-CCGTTAGATT-3ʹ
B) 5ʹ-AGGCTGCGAT-3ʹ and 5ʹ-AATCTAACGG-3ʹ
C) 5ʹ-TCCGACGCTA-3ʹ and 5ʹ-GGCAATCTAA-3ʹ
D) 5ʹ-TCCGACGCTA-3ʹ and 5ʹ-CCGTTAGATT-3ʹ

Question

Quizplus · Accepted Answer

Primers for PCR must flank the target sequence and be complementary to the template strands. The correct primers are 5ʹ-AGGCTGCGAT-3ʹ, which is complementary to the leading strand, and 5ʹ-AATCTAACGG-3ʹ, which is the reverse complement of 5ʹ-CCGTTAGATT-3ʹ, ensuring amplification of the target sequence.

Which Pair of Primers Will Amplify the Template DNA Sequence