Which method would be used to determine if drug treatment of a cell culture led to increased expression of mRNA?
A) Southern blotting
B) Northern blotting
C) Western blotting
D) Eastern blotting

Question

Quizplus · Accepted Answer

Northern blotting is a technique used to detect and quantify specific RNA molecules in a sample. By comparing the levels of mRNA in a drug-treated cell culture to those in a control group, it can be determined if the treatment led to an increase in mRNA expression. Southern blotting is used to detect specific DNA sequences, and Western blotting is used to detect specific proteins. Eastern blotting is not a commonly used technique.

Which Method Would Be Used to Determine If Drug Treatment