To clone genes, a plasmid vector should contain all of the following except
A) foreign DNA.
B) an origin of replication.
C) a restriction nuclease cut site.
D) an antibiotic resistance gene.

Question

Quizplus · Accepted Answer

Foreign DNA is what is being cloned, not a requirement of the plasmid vector itself. The plasmid vector needs an origin of replication to allow it to replicate within a host cell, a restriction nuclease cut site to allow the insertion of foreign DNA, and an antibiotic resistance gene to enable the selection of cells that have taken up the plasmid.

To Clone Genes, a Plasmid Vector Should Contain All of the Following