Which technique would be used to clone intact pieces of human DNA that are 400 kb pairs long?
A) An E. coli plasmid
B) Bacteriophage $\lambda$
C) Yeast artificial chromosomes
D) Polymerase chain reaction

Question

Quizplus · Accepted Answer

Yeast artificial chromosomes (YACs) are able to accommodate very large pieces of DNA, up to 1 Mb in size. YAC libraries have been used for genomic mapping and sequencing, gene identification, and disease gene studies. YACs are genetically stable and can be propagated in yeast, making them a useful tool for cloning large DNA fragments. A) An E. coli plasmid is not suitable for cloning such large fragments of DNA. B) Bacteriophage F1F1F1S1F1F1F10 is not a real bacteriophage and no information on its suitability for cloning large fragments of DNA is available. D) The polymerase chain reaction (PCR) is a powerful tool for amplifying specific DNA sequences, but it is limited to fragments of only a few hundred base pairs in length.

Which Technique Would Be Used to Clone Intact Pieces of Human