How is a DNA fragment containing a gene of interest generated for cloning?
A) The gene with a cloning vector is ligated.
B) Agarose gel electrophoresis takes place.
C) The polymerase chain reaction (PCR) amplifies the DNA fragment from the genome.
D) A DNA polymerase specific for the fragment is used to make copies.
E) DNA fingerprinting allows the fragment to be copied from the chromosome.

Question

Quizplus · Accepted Answer

The polymerase chain reaction (PCR) is a technique used to amplify a specific DNA fragment from the genome, making it a common method for generating a DNA fragment containing a gene of interest for cloning.

How Is a DNA Fragment Containing a Gene of Interest