During DNA cloning, why is the DNA of interest inserted into the middle of the lacZ⁺coding sequence in the plasmid vector? A) It makes the bacteria resistant to the antibiotic. B) It allows researchers to distinguish bacteria that contain the plasmid from those that do not. C) It aids scientists in developing new antibiotic treatments. D) It allows researchers to distinguish bacteria that contain the recombinant plasmid from those that contain just the vector. E) It makes the bacteria produce more clones.

Question

Quizplus · Accepted Answer

Inserting the DNA of interest into the lacZ gene disrupts its function, allowing researchers to use blue-white screening to distinguish between bacteria that have taken up the recombinant plasmid (white colonies) and those that only have the non-recombinant vector (blue colonies).

During DNA Cloning, Why Is the DNA of Interest Inserted