Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. Blue colonies on the plate contain bacteria with a ____ plasmid, meaning the lacZ⁺gene is ____. A) recombinant; deleted from the plasmid B) nonrecombinant; intact C) recombinant; intact D) nonrecombinant; disrupted by the inserted DNA fragment E) recombinant; disrupted by the inserted DNA fragment

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Quizplus · Accepted Answer

Blue colonies indicate that the lacZ gene is intact and functional, allowing the bacteria to metabolize X-gal and produce a blue color. This suggests that the plasmid has not been disrupted by the insertion of foreign DNA, making it nonrecombinant.

Bacteria Transformed with Plasmids Are Plated on Growth Medium Containing