How do scientists determine the annealing temperature used in PCR reactions? A) It is always 55^ᵒC because the DNA polymerase works best at this temperature. B) It is always 55^ᵒC because primers anneal best at lower temperatures. C) It is always 65^ᵒC because any other temperature would destabilize the DNA. D) It can vary from 55-65^ᵒC depending on the primers being used. E) It can vary from 55-65^ᵒC depending on the type of DNA polymerase being used.

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The annealing temperature in PCR reactions is determined based on the primers being used. It varies to optimize the binding of the primers to the DNA template, typically ranging from 55-65°C, depending on the primer's melting temperature.

How Do Scientists Determine the Annealing Temperature Used in PCR