The phases of each PCR cycle listed in order are ____.
A) denaturation, extension, and annealing
B) annealing, extension, and denaturation
C) extension, denaturation, and annealing
D) annealing, denaturation, and extension
E) denaturation, annealing, and extension

Question

Quizplus · Accepted Answer

The correct order of phases in a PCR cycle is denaturation (where the double-stranded DNA is heated to separate it into two single strands), annealing (where primers bind to the single-stranded DNA), and extension (where the DNA polymerase synthesizes the new DNA strand by extending from the primers).

The Phases of Each PCR Cycle Listed in Order Are