40. Identify the correct steps for cloning a gene of interest, then arrange them in the correct order. One of the steps below is left out of the answer. 1. Transform the bacteria.
2) Incubate the DNA fragments and cut cloning vector with DNA ligase.
3) Use restriction enzymes to cut the gene of interest and the cloning vector.
4) Spread bacteria on medium containing lactose and ampicillin.
5) Spread bacteria on medium containing X-gal and ampicillin.
A) 4, 3, 1, 5
B) 3, 2, 5, 1
C) 3, 2, 1, 5
D) 2, 3, 1, 5
E) 3, 2, 1, 4

Question

40. Identify the correct steps for cloning a gene of interest, then arrange them in the correct order. One of the steps below is left out of the answer. 1. Transform the bacteria.
2) Incubate the DNA fragments and cut cloning vector with DNA ligase.
3) Use restriction enzymes to cut the gene of interest and the cloning vector.
4) Spread bacteria on medium containing lactose and ampicillin.
5) Spread bacteria on medium containing X-gal and ampicillin.
A) 4, 3, 1, 5
B) 3, 2, 5, 1
C) 3, 2, 1, 5
D) 2, 3, 1, 5
E) 3, 2, 1, 4

Quizplus · Accepted Answer

The correct steps for cloning a gene of interest in order are: 3) Use restriction enzymes to cut the gene of interest and the cloning vector, 2) Incubate the DNA fragments and cut cloning vector with DNA ligase to join them, 1) Transform the bacteria with the recombinant DNA, and 5) Spread bacteria on medium containing X-gal and ampicillin to select for those that have taken up the gene of interest.

40 Identify the Correct Steps for Cloning a Gene of Interest