If a restriction endonuclease cuts a circular DNA three times, how many fragments should there be after the DNA is subjected to electrophoresis through an agarose gel?
A) 1
B) 2
C) 3
D) 4
E) 5

Question

Quizplus · Accepted Answer

When a circular DNA is cut three times by a restriction endonuclease, it will produce three fragments. These fragments can be separated by electrophoresis through an agarose gel, and will appear as three distinct bands on the gel.

If a Restriction Endonuclease Cuts a Circular DNA Three Times