RFLP screening during a chromosomal walk would permit the identification of a
A) DNA interval deleted in unrelated individuals who suffer from the same genetic disease.
B) point mutation in a gene that does not alter a restriction enzyme recognition sequence.
C) cDNA clone for the disease of interest.
D) clone expressing the mutant protein.

Question

Quizplus · Accepted Answer

RFLP screening involves identifying differences in restriction fragment patterns between individuals. If unrelated individuals suffering from the same genetic disease have a deleted DNA interval, RFLP screening could identify this as a difference in the restriction fragment pattern between affected and unaffected individuals. This would allow for the identification of the deleted interval and potentially the gene responsible for the disease. RFLP screening would not be useful for identifying the other options listed.

RFLP Screening During a Chromosomal Walk Would Permit the Identification