Selection of a Yeast Artificial Chromosome (YAC) vector for the cloning of human genomic DNA would greatly expedite
A) chromosomal walking.
B) efficient translation of human proteins.
C) expression screening.
D) differential screening.

Question

Quizplus · Accepted Answer

Yeast artificial chromosomes (YACs) are capable of carrying large DNA fragments, up to 1 megabase in size, making them ideal for cloning large sections of genomic DNA. YACs also permit chromosomal walking, a technique for locating a gene of interest by stepping through sections of DNA, which is useful for genome mapping and sequencing. YACs do not have any inherent advantages for protein expression or expression screening, and while they can be used for differential screening, their greatest strength is in their ability to handle very large DNA fragments.

Selection of a Yeast Artificial Chromosome (YAC) Vector for the Cloning