Which stage in the process of gene cloning are the restriction endonucleases utilized?
A) isolation of mRNA
B) synthesis of double-stranded cDNA
C) insertion of the cDNA into the vehicle
D) transformation
E) expression of the cDNA in the bacterial cell

Question

Quizplus · Accepted Answer

Restriction endonucleases are used during the insertion of cDNA into the vector. The restriction endonucleases are used to cleave the vector and insert the cDNA at the site of the cleavage. This process is called restriction enzyme digestion, and it allows for the insertion of foreign DNA into the vector.

Which Stage in the Process of Gene Cloning Are the Restriction