Chromosome walking
A) finds overlapping cDNAs.
B) allows long contiguous regions of DNA to be isolated.
C) requires the synthesis of artificial chromosomes.
D) requires somatic cell hybridization.
E) involves the direct sequencing of genomic DNA.

Question

Quizplus · Accepted Answer

Chromosome walking involves the step-by-step identification and sequencing of DNA fragments that are adjacent to a known sequence. This allows long contiguous regions of DNA to be isolated, hence option B is the correct answer. The method does not necessarily involve finding overlapping cDNAs (option A), synthesizing artificial chromosomes (option C), or somatic cell hybridization (option D), and it may or may not involve direct sequencing of genomic DNA (option E).

Chromosome Walking