In the Sanger dideoxy method of DNA sequencing, the dideoxy analog
A) allows specific chemical cleavage of the DNA strand.
B) is a component of the primer.
C) causes termination of DNA synthesis.
D) cannot be utilized for DNA synthesis by DNA polymerase I.
E) lacks a 5' phosphate group.

Question

Quizplus · Accepted Answer

The dideoxy analogs lack the 3'-OH group needed for the formation of a phosphodiester bond, so when they are incorporated into a growing DNA chain during synthesis, they cause termination of DNA synthesis. This allows for the determination of the sequence of the DNA strand.

In the Sanger Dideoxy Method of DNA Sequencing, the Dideoxy