The Gen-Probe AccuProbe system uses the following technology principle:
A) When two solutions are combined and hybridization occurs between the probe and the target, then the tube is centrifuged to detect the precipitated hybridized target and probes.
B) A single-stranded, chemiluminescent-labeled DNA probe is designed to hybridize to the target organism's ribosomal RNA (rRNA) , forming a DNA:RNA duplex.
C) A double-stranded, bioluminescent-labeled DNA probe is designed to hybridize to the target organism's DNA, forming a DNA:DNA duplex.
D) A single-stranded, chemiluminescent-labeled RNA probe is designed to hybridize to the target organism's ribosomal RNA (rRNA) , forming an RNA:RNA duplex.
Correct Answer:
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Q2: The variables that affect the outcome of
Q4: Many hybridization assay conditions are based on
Q5: The target nucleic acid strand is:
A) The
Q6: What types of compounds are used for
Q7: The steps for performing the Northern blot
Q8: A Northern blot is used to detect:
A)
Q9: The function of the probe is to:
A)
Q10: Nucleic acid hybridization is:
A) Coupling of complementary
Q11: The principle of solid support hybridization (often
Q14: Who received the Nobel Prize in Chemistry
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