Many hybridization assay conditions are based on the expectation that a probe:
A) Will quickly react with the complementary target sequence.
B) Is not sterically hindered in the reaction
C) Has exact complementarity to the target nucleic acid
D) Will not require any oligonucleotides to attach to the target

Question

Quizplus · Accepted Answer

Many hybridization assay conditions are based on the expectation that a probe has exact or near-exact complementarity to the target nucleic acid.This will not always be the case.When conditions are stringent,exact matches between the probe and target will hybridize first,whereas mismatches form duplexes more slowly.

Many Hybridization Assay Conditions Are Based on the Expectation That