The steps for performing the Northern blot test include all the following EXCEPT:
A) RNA is separated in an agarose gel.
B) The separated RNA is transferred from the agarose gel onto a membrane.
C) The membrane is then transferred to a gel so that the RNA can be immobilized in the gel.
D) The immobilized RNA is detected with a probe that hybridizes to the RNA species of interest.

Question

Quizplus · Accepted Answer

Northern blots may be used to determine the size of particular RNA molecules and to semiquantitate the amount of a particular RNA transcript.The actual procedure of the Northern blot is similar to that of the Southern blot; RNA is separated in an agarose gel,transferred to a membrane,immobilized,and detected with a probe that hybridizes to the RNA species of interest.Detection is also accomplished to the type of label on the probe.

The Steps for Performing the Northern Blot Test Include All