The steps for performing the Southern blot test include all the following EXCEPT:
A) After the labeled probe is hybridized to the specific target, the DNA is electrophoresed again, then dried and read.
B) The DNA is first digested with restriction enzymes.
C) Once digested, the DNA is separated and immobilized onto a solid membrane.
D) Labeled probe is hybridized to the specific DNA sequence.

Question

Quizplus · Accepted Answer

The DNA is first digested because chromosomal DNA is too large to separate in an agarose gel.Once the DNA has been separated and immobilized onto a solid membrane,labeled probe is hybridized to the specific target DNA sequence and detected.

The Steps for Performing the Southern Blot Test Include All