The principle of solid support hybridization (often called blotting)is:
A) The target sequence is part of the liquid support, and the probe, which is attached to solid support, hybridizes to the target.
B) All the target nucleic acids are gathered in one spot on the electrophoresis gel. Then all the probes are flooded in the same area and produce a blot of duplexes.
C) The target sequence is part of the solid support, and the probe, which is in solution, hybridizes to the target.
D) The probe is electrophoresed in agar, then transferred to filter paper. The antibody-based probe is then applied to the filter paper, and blots appear where there is binding.

Question

Quizplus · Accepted Answer

In solid support hybridization (a technique often called "blotting"),the target nucleic acid is transferred and immobilized to a membrane,composed of either nitrocellulose or nylon.The solid membrane is often pretreated to reduce nonspecific probe binding to the membrane itself.Labeled probe is then hybridized to the immobilized nucleic acid,and washing steps are utilized to remove excess probe and clarify the signal.

The Principle of Solid Support Hybridization (Often Called Blotting)is