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Concepts of Genetics

Biology

Quiz 5 :

Chromosome Mapping in Eukaryotes

Quiz 5 :

Chromosome Mapping in Eukaryotes

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Assume that a cross is made between AaBb and aabb plants and that all the offspring are either AaBb or aabb. These results are consistent with the following circumstance:
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A

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Diagram chromosomal events that will ultimately result in the segregation of alleles (A and a) during meiosis II rather than meiosis I.
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img

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The genes for mahogany eyes and ebony body are approximately 25 map units apart on chromosome III in Drosophila. Assume that a mahogany-eyed female was mated to an ebony-bodied male and that the resulting F1 phenotypically wild-type females were mated to mahogany, ebony males. Of 1000 offspring, what would be the expected phenotypes, and in what numbers would they be expected?
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Answer:

mahogany = 375; ebony = 375; wild-type = 125; mahogany-ebony = 125.

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Who was the student in Morgan's laboratory who first realized that the sequence of genes could be determined by Morgan's proposal that two genes located relatively close to each other are less likely to form chiasma between them than if the two genes were relatively far apart?
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The phenomenon in which one crossover decreases the likelihood of crossovers in nearby regions is called
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Based on the classic experiments of Creighton and McClintock with maize, crossing over involves a physical exchange between chromatids. What particular chromosomal characteristic allowed their experiments to succeed?
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Under what circumstance might two loci be on the same chromosome but behave as if independently assorting in crosses?
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At what stage of the meiotic cell cycle and during what chromosomal configuration does crossing over occur?
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Assume that there are 12 map units between two loci in the mouse and that you are able to microscopically observe meiotic chromosomes in this organism. If you examined 200 primary oocytes, in how many would you expect to see a chiasma between the two loci mentioned above?
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Assume that a cross is made between AaBb and aabb plants and that the offspring occur in the following numbers: 106 AaBb, 48 Aabb, 52 aaBb, 94 aabb. These results are consistent with the following circumstance:
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Phenotypically wild-type F1 female Drosophila, whose mothers had light eyes (lt) and fathers had straw (stw) bristles, produced the following offspring when crossed to homozygous light-straw males: img Compute the map distance between the light and straw loci.
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Methods for determining the linkage group and genetic map in humans involve which of the following?
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In Drosophila, assume that the gene for scute bristles (s) is located at map position 0.0 and that the gene for ruby eyes (r) is at position 15.0. Both genes are located on the X chromosome and are recessive to their wild-type alleles. A cross is made between scute-bristled females and ruby-eyed males. Phenotypically wild-type F1 females were then mated to homozygous double mutant males, and 1000 offspring were produced. Give the phenotypes and frequencies expected.
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Assume that the genes for tan body and bare wings are 15 map units apart on chromosome II in Drosophila. Assume also that a tan-bodied, bare-winged female was mated to a wild-type male and that the resulting F1 phenotypically wild-type females were mated to tan-bodied, bare-winged males. Of 1000 offspring, what would be the expected phenotypes, and in what numbers would they be expected?
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Assume that a cross is made between AaBb and aabb plants and that the offspring fall into approximately equal numbers of the following groups: AaBb, Aabb, aaBb, aabb. These results are consistent with the following circumstance:
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What term is applied when two genes fail to assort independently, that is, they tend to segregate together during gamete formation?
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What two terms apply to the fusion of cultured human and mouse cells that produces cell lines that are useful in assigning a gene to a particular human chromosome?
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What is the relationship between the degree of crossing over and the distance between two genes?
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Assume that investigators crossed a strain of flies carrying the dominant eye mutation Lobe on the second chromosome with a strain homozygous for the second chromosome recessive mutations smooth abdomen and straw body. The F1 Lobe females were then backcrossed with homozygous smooth abdomen, straw body males, and the following phenotypes were observed: smooth abdomen, straw body 820 Lobe 780 smooth abdomen, Lobe 42 straw body 58 smooth abdomen 148 Lobe, straw body 152 a) Give the gene order and map units between these three loci. b) What is the coefficient of coincidence?
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What relatively recent scientific advancement has made mapping by linkage or classical genetic mapping approaches virtually obsolete?
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