Which one of the following statements about Southern blot analysis is true?
A) It will identify which RNA in a population is complementary to a specific DNA probe.
B) It is used to purify a gene by electrophoretic separation from a population of genes.
C) It is useful for repetitive DNA but not unique sequence DNA.
D) It is used to amplify a specific DNA sequence in vitro.
E) It identifies a specific fragment of DNA based on complementarity to a radioactive DNA probe.

Question

Quizplus · Accepted Answer

Southern blot analysis uses restriction enzymes to cut DNA fragments, which are then separated by gel electrophoresis based on size. The DNA fragments are then transferred to a membrane and probed with a labeled DNA probe that is complementary to the target sequence. The probe will bind to the target sequence on the membrane, allowing identification and visualization of a specific fragment of DNA based on complementarity to the radioactive DNA probe. This technique is highly specific and sensitive, allowing for the detection of small amounts of DNA.

Which One of the Following Statements About Southern Blot Analysis