To obtain recombinant DNA clones that encode a specific protein, a high affinity antibody to that protein can be utilized to screen
A) a cDNA library in pBR.
B) a cDNA library in an expression vector.
C) a genomic DNA library cloned into a YAC vector.
D) a cDNA library cloned into a YAC vector.
E) a genomic DNA library cloned into a phage vector.

Question

Quizplus · Accepted Answer

A cDNA library in an expression vector would be the best choice because it contains clones of cDNA that are expressed as proteins, which can be screened using a high affinity antibody to the specific protein of interest. This would allow for the isolation of recombinant DNA clones that produce the desired protein.

To Obtain Recombinant DNA Clones That Encode a Specific Protein