DNA sequencing by the Sanger technique makes use of ____________for chain termination.
A) dNTPs
B) dideoxynucleotides
C) AZT
D) DNA polymerase

Question

Quizplus · Accepted Answer

The Sanger technique utilizes dideoxynucleotides (ddNTPs) to terminate chain elongation during DNA sequencing. These ddNTPs lack the 3' hydroxyl group necessary for the formation of the phosphodiester bond between nucleotides, which prevents further elongation of the DNA strand.

DNA Sequencing by the Sanger Technique Makes Use of ____________For