In the preparation of labeled probes, which steps do nick-translation and random oligonucleotide priming have in common?
A) Labeling reactions are initiated by synthetic oligonucleotides.
B) The incorporation of labeled precursor involves an in vitro DNA replication reaction.
C) Nuclease activity can degrade newly synthesized, labeled DNA.
D) They both incorporate -labeled ribonucleotides.

Question

Quizplus · Accepted Answer

Both nick-translation and random oligonucleotide priming involve incorporating labeled precursors into DNA through an in vitro DNA replication reaction. Nuclease activity can also be a concern for both methods, but the other choices do not apply to both techniques.

In the Preparation of Labeled Probes, Which Steps Do Nick-Translation