In Southern Blot Hybridization Analysis

In Southern blot hybridization analysis

A) reverse transcriptase is used to synthesize a radioactive nucleic acid probe
B) nick translation is used to obtain a protein-derived probe.
C) DNA is denatured and lifted from the agar surface with a nitrocellulose filter.
D) restriction fragments containing a specific nucleotide sequence can be identified.
E) DNA fragments are resolved on a CsCl density gradient.