RFLP linkage analysis followed by chromosome walking is used to find disease-causing genes. Which of the following would be a good indication that the disease-causing gene has been found by this analysis?
A) The gene is at least one cM from the RFLP.
B) The gene is homozygous in the affected individual.
C) The gene contains a nonsense mutation.
D) The gene is heterozygous in the individual.
E) The gene contains numerous polymorphisms.

Question

Quizplus · Accepted Answer

Finding a gene with a nonsense mutation during RFLP linkage analysis and chromosome walking is a good indication that the disease-causing gene has been found. Nonsense mutations often result in a premature stop codon, leading to a truncated and usually nonfunctional protein, which can cause disease.

RFLP Linkage Analysis Followed by Chromosome Walking Is Used to Find