Segments of pure samples of identical, relatively short DNA molecules from plasmids can be subdivided into smaller pieces by
A) Southern blot analysis.
B) colony hybridization.
C) restriction enzyme digestion.
D) agarose gel electrophoresis.
E) ethidium bromide treatment.

Question

Quizplus · Accepted Answer

Restriction enzyme digestion is a commonly used method for cutting DNA into smaller pieces. The enzyme recognizes and cuts at specific nucleotide sequences, allowing for precise fragmentation of the DNA. Southern blot analysis, colony hybridization, agarose gel electrophoresis, and ethidium bromide treatment are techniques used for analyzing DNA fragments, but do not involve the physical fragmentation of the DNA.

Segments of Pure Samples of Identical, Relatively Short DNA Molecules