Screening a recombinant DNA library using an 125I-labeled antibody prepared against a eukaryotic protein
A) requires that cDNA inserts have been used in the library being screened.
B) requires the utilization of vector sequences that provide high efficiency translation of the cloned insert DNA including signals for initiation and termination of translation.
C) requires the utilization of vector sequences that provide high efficiency transcription of the cloned insert DNA including signals for initiation and termination of transcription.
D) will isolate any gene that encodes a protein product recognized by the antibody.
E) all of the above statements are true.

Question

Quizplus · Accepted Answer

Screening a recombinant DNA library with a labeled antibody (in this case, using $^{125}I$) to identify a eukaryotic protein requires several conditions to be met, which are covered by all the options provided. The cDNA inserts (A) ensure that the library contains the coding sequences for eukaryotic proteins without introns, which can complicate expression in prokaryotic systems. High efficiency translation (B) and transcription (C) of the cloned insert DNA are crucial for producing enough protein to be detected by the antibody. This process involves appropriate vector sequences that include signals for both processes. Finally, this method (D) indeed isolates genes encoding proteins that are recognized by the specific antibody used, making all the statements true.

Screening a Recombinant DNA Library Using an 125I-Labeled Antibody Prepared