Allele-specific oligonucleotide (ASO) analysis is used primarily for
A) identification of point mutations.
B) detection of deleted exons.
C) linkage analysis.
D) detection of trinucleotide repeat expansions.

Question

Quizplus · Accepted Answer

Allele-specific oligonucleotide (ASO) analysis is most commonly used for the identification of point mutations, as it involves the use of short, single-stranded DNA sequences that are complementary to a specific nucleotide sequence in the target gene. These oligonucleotides can be specifically designed to detect a known single nucleotide polymorphism (SNP) or point mutation in the gene of interest, allowing for a sensitive and specific detection method. ASO analysis is less commonly used for detection of deleted exons, linkage analysis, or trinucleotide repeat expansions.

Allele-Specific Oligonucleotide (ASO) Analysis Is Used Primarily for